first_imgCreationism conference at large U.S. research university stirs uneaseA creationist conference set for a major research campus—Michigan State University in East Lansing—is creating unease among some of the school’s students and faculty, which includes several prominent evolutionary biologists. The 1-day conference, called the Origin Summit, will include eight workshops, including discussion of how evolutionary theory influenced Adolf Hitler’s worldview, why “the big bang is fake,” and why “natural selection is NOT evolution.”Avalanche on an asteroid, due to close pass with EarthSign up for our daily newsletterGet more great content like this delivered right to you!Country *AfghanistanAland IslandsAlbaniaAlgeriaAndorraAngolaAnguillaAntarcticaAntigua and BarbudaArgentinaArmeniaArubaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBermudaBhutanBolivia, Plurinational State ofBonaire, Sint Eustatius and SabaBosnia and HerzegovinaBotswanaBouvet IslandBrazilBritish Indian Ocean TerritoryBrunei DarussalamBulgariaBurkina FasoBurundiCambodiaCameroonCanadaCape VerdeCayman IslandsCentral African RepublicChadChileChinaChristmas IslandCocos (Keeling) IslandsColombiaComorosCongoCongo, The Democratic Republic of theCook IslandsCosta RicaCote D’IvoireCroatiaCubaCuraçaoCyprusCzech RepublicDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEthiopiaFalkland Islands (Malvinas)Faroe IslandsFijiFinlandFranceFrench GuianaFrench PolynesiaFrench Southern TerritoriesGabonGambiaGeorgiaGermanyGhanaGibraltarGreeceGreenlandGrenadaGuadeloupeGuatemalaGuernseyGuineaGuinea-BissauGuyanaHaitiHeard Island and Mcdonald IslandsHoly See (Vatican City State)HondurasHong KongHungaryIcelandIndiaIndonesiaIran, Islamic Republic ofIraqIrelandIsle of ManIsraelItalyJamaicaJapanJerseyJordanKazakhstanKenyaKiribatiKorea, Democratic People’s Republic ofKorea, Republic ofKuwaitKyrgyzstanLao People’s Democratic RepublicLatviaLebanonLesothoLiberiaLibyan Arab JamahiriyaLiechtensteinLithuaniaLuxembourgMacaoMacedonia, The Former Yugoslav Republic ofMadagascarMalawiMalaysiaMaldivesMaliMaltaMartiniqueMauritaniaMauritiusMayotteMexicoMoldova, Republic ofMonacoMongoliaMontenegroMontserratMoroccoMozambiqueMyanmarNamibiaNauruNepalNetherlandsNew CaledoniaNew ZealandNicaraguaNigerNigeriaNiueNorfolk IslandNorwayOmanPakistanPalestinianPanamaPapua New GuineaParaguayPeruPhilippinesPitcairnPolandPortugalQatarReunionRomaniaRussian FederationRWANDASaint Barthélemy Saint Helena, Ascension and Tristan da CunhaSaint Kitts and NevisSaint LuciaSaint Martin (French part)Saint Pierre and MiquelonSaint Vincent and the GrenadinesSamoaSan MarinoSao Tome and PrincipeSaudi ArabiaSenegalSerbiaSeychellesSierra LeoneSingaporeSint Maarten (Dutch part)SlovakiaSloveniaSolomon IslandsSomaliaSouth AfricaSouth Georgia and the South Sandwich IslandsSouth SudanSpainSri LankaSudanSurinameSvalbard and Jan MayenSwazilandSwedenSwitzerlandSyrian Arab RepublicTaiwanTajikistanTanzania, United Republic ofThailandTimor-LesteTogoTokelauTongaTrinidad and TobagoTunisiaTurkeyTurkmenistanTurks and Caicos IslandsTuvaluUgandaUkraineUnited Arab EmiratesUnited KingdomUnited StatesUruguayUzbekistanVanuatuVenezuela, Bolivarian Republic ofVietnamVirgin Islands, BritishWallis and FutunaWestern SaharaYemenZambiaZimbabweI also wish to receive emails from AAAS/Science and Science advertisers, including information on products, services and special offers which may include but are not limited to news, careers information & upcoming events.Required fields are included by an asterisk(*)On Friday the 13th in April 2029, an asteroid named Apophis is expected to pass, with luck, within a hair’s breadth of Earth. The space rock, about as wide as three football fields, won’t do any damage to Earth, but the near miss could trigger tiny avalanches on the asteroid itself.Algal virus found in humans, slows brain activityIt’s not such a stretch to think that humans can catch the Ebola virus from monkeys and the flu virus from pigs. But now researchers have discovered that even a virus found in lowly algae can infect humans. Although the bug doesn’t make us sick, it does seem to slow certain brain activities.The seven creepiest science experimentsScience is amazing, but it can involve doing some pretty weird stuff. Just in time for Halloween, here are seven science experiments that are creepy, scary, or just plain gross!’Dance Your Ph.D.’ finalists announcedMeet the finalists for this year’s Dance Your Ph.D. contest! These 12 amazing teams of scientists turned their Ph.D. theses into dances and stand to win fame, eternal geek glory, and a trip to California. Tune in Monday, 3 November, to see the winners!Scientists grow tiny human stomachs in lab dishesResearchers have managed to get stem cells in the lab to grow into human stomachs smaller than a single pea. Until now, the human gut has been hard to study because it’s so different from those in typical model organisms like mice, so these tiny tummies open up a world of possibility.Been to an Ebola-affected country? Stay away, Louisiana saysEbola fears are interfering with the world’s premier scientific meeting on tropical diseases. This week, Louisiana state health officials asked anyone who has traveled to Liberia, Sierra Leone, or Guinea in the past 21 days, or has treated Ebola patients elsewhere, to stay away from the annual meeting of the American Society of Tropical Medicine and Hygiene, which begins on Sunday in New Orleans.See all of Science’s coverage of the Ebola outbreak, including Liberia’s Ebola progress, the latest vaccine news, and what the virus actually does.last_img read more

first_imgCity Touch’s victory in this year’s event avenged last year’s runner up positions in both the Men’s and Women’s Open divisions at the hands of Adelaide Uni. The Blacks again proved they were a force reaching both finals however it was City Touch’s turn in 2011.At the commencement of play on day two it was always going to be a battle between the two heavyweight clubs but a tight one with games going either way in pool play.The Women’s Open final lived up to all the hype with both Adelaide Uni and City unable to score prior to half-time. Shortly after half-time City Touch drew first blood and this lifted the young City team, who were able to run out winners 4-1 in the end and claim their maiden State Cup Women’s Open crown.An arm wrestle was predicted in the Men’s final as well and it was Adelaide Uni who flew out of the blocks scoring the opening touchdown in the first 30 seconds of the grand final. Not to be outdone, City immediately hit back levelling the encounter 1-all. Both sides traded early blows however at the break it was City who had built up a handy buffer of 5-2 in a frantic first half. Desperate to keep in the game Adelaide Uni scored first, however multiple scores from Mitch Annetts and Tim Glazebrook secured the title for City Touch who ended up running out comfortable winners in the end 8-4.With the two grand final victories under their belts City Touch Club was crowned 2011 SA Champion Affiliate (State Cup Champions).Flinders Touch also had reason to be proud claiming third position overall and having a great time in doing so. The guys and girls from Flinders also had reason to celebrate when Dave Harris was named Male Most Valuable Player of the tournamentKelly Rowe added the icing on the City Touch cake claiming the Women’s Open Most Valuable Player in what has been a stellar 2011 for the 18-year-old.Of course the officials also were rewarded with Phil Spencer being named Referee of the Tournament and also receiving an upgrade to Level 3 referee. Other referees to receive upgrades were Kate Bain and Denis Wooldrige who both were upgraded to Level 2.Both SA Heat and Alliance selectors were pleased with the tournament, with several players identified as potential representative players for both squads.Touch Football South Australia would like to congratulate and thank all players and affiliates who participated and make special mention to all the referees and selectors who once again did an outstanding job.Please also find attached the November edition of the TFSA Newsletter, Touch Shorts. Related Filestfsa_newsletter_nov_2011-pdflast_img read more

first_img Live Out Your Westworld Dreams at Casteel Creek Retreat The three upper floors of Fjord House are private and home to the financial firm Kirk Kapital, whose owners are direct descendants of the founder of LEGO (suddenly the stacked brick construction makes sense, doesn’t it?). These office spaces were also designed around the circle. Rooms are round and custom circular light fixtures and furnishings were specially created by Eliasson. Thanks to the arch cutouts, these spaces are flooded with light from all sides, creating bright, beautiful offices for the financial firm. It all makes for a visually stunning piece of architecture that seems straight out of the future. And while it may be home to a business, we still think Fjordenhus is the perfect spot for a blade runner looking to hide out. This All-Black Cabin is Like a Pair of Binoculars Taking in the Canadian Wilderness Every now and then, a piece of architecture seems so outlandish, so futuristic, you are positive it is a leftover set from some sci-fi adventure flick. Frank Lloyd Wright’s Ennis House was so advanced when it was designed back in 1924 that Ridley Scott thought it was the perfect spot for a 1980s movie set in the year 2019. While Ennis House was home to Rick Deckard in the original Blade Runner, we think Fjordenhus would have made the perfect hideout for him in Blade Runner 2049.Fjordenhus, or Fjord House, is a series of tubes (no really) rising out of the harbor of Vejle, Denmark. The building sits at just over 91 feet in height and is accessed via a real-life drawbridge. Comprised of four circular structures that are interconnected, Fjord House was built from 970,000 multi-colored bricks. In all, 15 different tones of unglazed brick were used, creating a building that, from the outside, seems to change color throughout the day.Once you cross the drawbridge into Fjord House, you are greeted with a public art gallery in a grand, open-air vestibule. The work of architect and artist Olafur Eliasson, the pieces are intended to be a play on the changing colors and ephemeral feel of water and light. Also on the main floor are two viewing platforms just above the water that are intended to be art installations in their own right.Throughout the building, the circle is king. While some walls are circular due to the shape of the building, in other areas, parts of the circles were cut away to create concave walls that swoop down into the water below. Stunning circular staircases rise to the upper floors and wrap around circular vestibules which look down to the water. Most dramatically, large arches were cut into the exterior walls, revealing the different (again, circular) floors of the building and allowing large windows to be put in. 1 of 4 This Little House in Washington Has Big Personality center_img For Sale: A Treehouse in the Suburbs Designed by John Lautner Editors’ Recommendations Previous Next Shark House’s Dramatic Angles Have Serious Bite last_img read more

first_img PICA Chief Executive Officer, Andrew Wynter, said the services will initially be available in Kingston, and, thereafter, “we (will) look at all of our locations… over the next few months… (to determine how) these services are going to be (further) rolled out”. The Passport, Immigration and Citizenship Agency’s (PICA) has introduced a drop box and courier service at its head office on Constant Spring Road in Kingston. Story Highlights It will be complemented by a courier service, being done in partnership with DHL Jamaica Limited, which will enable clients to arrange for the delivery of their documents after making the requisite payments. The Passport, Immigration and Citizenship Agency’s (PICA) has introduced a drop box and courier service at its head office on Constant Spring Road in Kingston.The drop box will facilitate speedier passport renewals by eliminating the need for clients to join long queues to have documents processed.It will be complemented by a courier service, being done in partnership with DHL Jamaica Limited, which will enable clients to arrange for the delivery of their documents after making the requisite payments.At the launch at the Police Officers’ Club in St. Andrew on Thursday, December 14, National Security Minister, Hon. Robert Montague, welcomed the initiatives.He lauded PICA for this “innovative idea” aimed at improving customer service delivery.“I urge everyone to make use of the drop box… and if you have ideas or suggestions… drop them in the box… . We will take them on,” he noted.PICA Chief Executive Officer, Andrew Wynter, said the services will initially be available in Kingston, and, thereafter, “we (will) look at all of our locations… over the next few months… (to determine how) these services are going to be (further) rolled out”.Mr. Wynter also advised that the online passport application system and customs and immigration (C5) declaration form will be launched soon.The latter service will enable persons travelling back to Jamaica to complete the form ahead of their arrival.“These… and a number of other initiatives that we are working on… are (intended) to assist you, our customers, passengers and fellow citizens of Jamaica to enjoy the products and services being offered by the Passport, Immigration and Citizenship Agency,” he added.Commercial Manager at DHL, Venice Williamson, said the firm was “extremely ecstatic” to be partnering with PICA.“We think that this is a very forward-thinking move on the part of PICA, and we are really and truly very humbled to be a part of this initiative,” she further said.The DHL executive gave the company’s undertaking to safeguard all documents and packages entrusted to its custody, and assured that clients will be able to keep track of their movement.“We aim to make it a very secure, reliable and extremely seamless process. We look forward to growing from strength to strength as we move through the different phases of this partnership,” Ms. Williamson added.last_img read more

first_imgShe also plans to perform a selection of those songs on the tour. Sept. 19 – Hamilton, FirstOntario Concert Hall The Canadian tour is an extension of three shows recently announced in Australia. Sept. 14 – St. John’s, Mile One Centre “Constant Craving” is the biggest hit of lang’s career and helped to skyrocket her to a new level of fame. A gala performance at the National Arts Centre in Ottawa on Sept. 16 will also feature the NAC’s orchestra as part of the show. Aug. 20 – Edmonton, Northern Alberta Jubilee Auditorium Login/Register With: The 23 dates begin with two nights in Victoria in August before lang heads across the country with stops in every province along the way. Advertisement Sept. 7 – Moncton, N.B., Casino New Brunswick Aug. 27 – Winnipeg, Burton Cummings Theatre Twitter LEAVE A REPLY Cancel replyLog in to leave a comment Aug. 17 – Vancouver, Orpheum Theatre Aug. 30 – Toronto, Sony Centre for the Performing Arts Aug. 19 – Edmonton, Northern Alberta Jubilee Auditorium Advertisement Aug. 26 – Regina, Conexus Arts Centre But lang has also garnered international acclaim for her other works, including the 2004 covers album “Hymns of the 49th Parallel,” which featured songs from legendary Canadian artists like Joni Mitchell, Leonard Cohen and Neil Young. Aug. 23 – Calgary, Southern Alberta Jubilee Auditorium Sept. 11 – Sydney, N.S., Centre 200 Advertisement Sept. 8 – Charlottetown, Eastlink Centre Charlottetown Sept. 17 – Ottawa, National Arts Centre Aug. 22 – Saskatoon, TCU Place A full list of dates: Sept. 9 – Halifax, Scotiabank Centre Sept. 2 – Montreal, Place des Arts – Salle Wilfrid-Pelletier Aug. 12 – Victoria, Royal Theatre Facebook Four-time Grammy winner k.d. lang will mark the success of her 1992 hit single “Constant Craving” and its album “Ingenue” with a 17-city Canadian tour. Sept. 16 – Ottawa, National Arts Centre Fundraising Gala (with NAC orchestra) Aug. 16 – Vancouver, Orpheum Theatre Aug. 24 – Calgary, Southern Alberta Jubilee Auditorium Aug. 31 – Toronto, Sony Centre for the Performing Arts Sept. 3 – Quebec City, Grand Theatre de Quebec Tickets for the Ingenue Redux Tour go on sale to fan club members March 2 while a general sale begins on March 3. Aug. 13 – Victoria, Royal Theatrelast_img read more

first_imgWILMINGTON, Vt. – A private Vermont ski resort that was the setting for “The Bachelor Winter Games” on ABC has been shut down for failing to pay taxes.The resort missed a required payment to the state and was ordered to close until it is paid, Meridith Dennes, a spokeswoman for The Hermitage Club, said Monday. “We are working diligently to secure the funds to allow us to open for this coming weekend.”The members-only resort owes more than $1 million in back taxes and recently laid off about 80 people, citing poor weather and cash-flow issues.Last month, a bank filed a foreclosure notice on several of the resort’s properties, saying the club had defaulted on more than $16 million in loans.State employees were posting public notices on the doors of the resort Monday afternoon, and the company was not allowed to open until it is able to come up with enough money to make its payments to the tax department, said Vermont Tax Commissioner Kaj Samsom.The Hermitage Club hosted “The Bachelor Winter Games,” a four-episode spin-off of “The Bachelor” that ran in conjunction with the Olympics.last_img read more

first_imgGILLAM, M.B. – RCMP to scale back search for Kam McLeod and Bryer SchmegelskyOver the next week, the search will be scaled down.  The majority of RCMP and Military personnel will leave Gillam with some members remaining in the community to continue the search.  The search for the suspects and the investigation into the deaths of Lucas Folwer, Chynna Deese and Leonard Dyck will continue.Assistant Commissioner Jane MacLatchy said during a press conference Wednesday “When searching for people in vast, remote rugged locations it’s always a possibility they will not be immediately located.” Tactical police have returned to spot where fugitives’ torched car was found – officer tells us they’re trying a new technique here – others in full camo gear standing by beside their vehicle.@9NewsAUS @TheTodayShow— Alexis Daish (@LexiDaish) July 31, 2019 The RCMP are still considering that the suspects may have had help to leave the area.  The RCMP is reminding the public to remain vigilant and report anything suspicious to your local police department.The RCMP and Canadian Military searched 11,000 kilometres in northern Manitoba for Bryer Schmegelsky and Kam McLeod.  They used what the RCMP called some of the most advanced technology possible to find the suspects.Even with this effort, there have been no confirmed sightings of the suspects.PICS: Police tell us today they’re working on the ground retracing steps from where Mcleod and Schmegelsky torched their car here outside of Gillam. There’s a scorched set of keys left behind.@9NewsAUS @TheTodayShow— Alexis Daish (@LexiDaish) July 31, 2019center_img The search near Gillam has been underway since July 22, 2019, and to date has not produced any sightings of Bryer Schmegelsky and Kam McLeod.The RCMP say they have checked over 500 homes in the Gillam and Fox Lake Cree Nation.  Investigators have now received over 260 tips in the past seven days. None have established that the suspects are outside of the Gillam area.Bryer Schmegelsky and Kam McLeod have been charged in connection with the death of Leonard Dyck near Dease Lake and are suspected of also killing Lucas Folwer and Chynna Deese.  The RCMP have not released details about when the pair will be officially charged in the deaths near the Liard Hot Springs of Folwer and Deese.last_img read more

The new study – a joint effort by the UN Foundation (UNF), the International Centre for Trade and Sustainable Development and the Energy and Security Group – examines bioenergy’s potential in the eight nations of the Economic and Monetary Union of West Africa (UEMOA), which comprises Benin, Burkina Faso, Côte d’Ivoire, Guinea-Bissau, Mali, Niger, Senegal and Togo.“The development, use, and commercialization of bioenergy offer UEMOA member countries vital economic, social, and environmental opportunities for transforming rural areas,” the report noted.If both produced and consumed locally, it could be a crucial tool used to address poverty, it added.“However, a strong policy framework at the local, national, and regional levels is required to ensure that these benefits are realized, shared equitably, and that negative impacts are minimized,” the new publication said.Policies must concentrate on the impact of bioenergy development on food security, the environment and the agricultural production system.The eight UEMOA nations “possess a rich resource base that can be sustained by a combination of good policies and practices to expand the production of and access to food, fuel, and fibre,” according to the report.“Undertaking these strategies to improve agriculture and forest productivity, protect watersheds, and produce bioenergy should also strengthen their ability to adapt to climate change.” 16 October 2008Sustainable bioenergy is a weapon that can be used by West African nations to combat poverty, according to a new United Nations-backed report released today. read more

The MECCA Mountain Bike Festival recently came to Green River for its 33rd year running.The day started with registration and two different warm up rides, one to the Athena Trail and the other to the sculpture in Green River. When riders returned, the guided rides started. With many trails to choose from, MECCA offered guides to take participants through trails in the Green River area.The night ended with a tribute to the founding member of the MECCA Mountain Bike Club, Lamar “Sheriff” Guymon, who passed away Oct. 12. Tribute was also given to Gale Anderson, a long-time MECCA supporter, who passed away April 14.MECCA has been running for 33 years and many who have worked as part of the committee have seen many of the same families return year after year. Some families have been attending the festival for 30 years and now bring their grandchildren with them. read more

Share this:Click to share on Twitter (Opens in new window)Click to share on Facebook (Opens in new window)RelatedMan killed execution-style in T&T KFC outletMay 15, 2017In “latest news”Miner fatally stabbed after refusing to give attacker alcoholMay 10, 2019In “Crime”‘Handy man’ stabbed to death during argument – attacker surrenders to PoliceJuly 4, 2016In “Crime” A 30-year-old man of Angoys Avenue, New Amsterdam, Berbice was stabbed shortly after he ran into a Police Station to seek refuge from his attacker.Reports are that an argument erupted between the man, who is said to be a pork knocker, and his attacker at the New Amsterdam Market.In a bid to escape, the man ran some 300 meters to the Traffic Department of the New Amsterdam’s Central Police Station while his attacker was in hot pursuit on a motorcycle.The victim made it into the Police Station, but his attacker rode his motorcycle into the building, crashing into the door. The loud impact reportedly sent officers dashing for cover.Armed with a knife, the 35-year-old attacker continued his pursuit of the pork knocker.The two men reportedly ran around several sections of the Police Station until the victim escaped outside, again with his attacker in pursuit.It was outside the Traffic Department the attacker stabbed the victim to his chest. A Police Officer then picked up a stick and hit the attacker.INews understands that the victim was recently released from prison. He was reportedly serving time for injuring the very man who stabbed him.Reports are that the feud between the men stemmed from a relationship both of them share with the same woman. Both men were arrested and are in custody as the investigation continues. read more

first_imgLONG BEACH — Razor clam digging is scheduled for Jan. 20-21 and Feb. 18-19 at selected beaches along the Washington coast.Final approval of the digging will depend on marine toxin tests showing the clams are safe to eat.Digging on Jan. 20-21 will be at Long Beach, Twin Harbors, Copalis and Mocrocks. The Feb. 18-19 dig will be at Long Beach, Twin Harbors and Mocrocks.The Washington Department of Fish and Wildlife announced the schedule on Tuesday. Digging will be allowed between midnight and noon.Low tides are at 4:28 p.m. (-0.5 feet) on Jan. 20 and 5:17 p.m. (-0.8 feet) on Jan. 21. Low tides are 4:13 p.m. (0.0 feet) and 5 p.m. (-0.2 feet) on Feb. 18 and 19, respectively.Additional digs in spring will be announced once a catch assessment is completed for the season through February, said Dan Ayres, coastal shellfish manager.last_img read more

first_imgChennai: Madras High Court judge Justice S Vaidyanathan on Tuesday withdrew his controversial observation that co-education in Christian institutes was “highly unsafe” for the future of girl children. The judge had made the observation recently when refusing to quash a show-cause notice issued to a Madras Christian College professor facing sexual harassment charge from at least 34 girl students pursuing third-year Zoology course at the college. Also Read – One arrested for firing outside Satna college in Madhya Pradesh Advertise With Us “There is a general feeling among parents of students, especially female students, that co-educational study in Christian institutions is highly unsafe for the future of their children,” Justice Vaidyanathan had said in his order dismissing the plea of Assistant Professor Samuel Tennyson. The judge had also said that Christian missionaries always came under attack for one issue or the other. Also Read – Abrogation Of Article 370 Carried Out In Inhuman Way: Urmila Matondkar Advertise With Us “In the present era, there are several accusations against them for indulging in the compulsory conversion of people of other religions into Christianity… Though they impart good education, their preaching of morality will be a million-dollar question,” Justice Vaidyanathan had added. Christian organisations, including the Tamil Nadu Bishops Council, and various sections of society had expressed concern over the observation of the Judge in the order. When the case came up on Tuesday, mention was made by John Jackria, counsel for the Madras Christian College, seeking withdrawal of the controversial observation. The judge, who acceded to the mention, said he was withdrawing para 32 of the order.last_img read more

first_img Journal information: Proceedings of the National Academy of Sciences (—Gene expression plays a central role in the orchestration of virtually all cellular processes. While inducible promoters have proven invaluable in understanding regulatory networks by modifying gene expression levels, their use has faced some shortcomings. Specifically, their utility is constrained to research studying relatively short-term, population-scale effects. Recently, however, scientists at Institut National de Recherche en Informatique et Automatique (INRIA) and at Centre National de la Recherche Scientifique (CNRS), France, have demonstrated that implementing an external feedback loop allows single-cell gene expression to be accurately and tightly controlled over many generations. The team accomplished this by developing a real-time, closed-loop control gene expression platform integrating microscopy, microfluidics, and original software for automated imaging, quantification, and model predictive control. They state that their study demonstrates long-term control with both time-constant and time-varying target expression profiles, at the population and single-cell levels, shows that real-time control can limit the effects of gene expression stochasticity, and anticipate that their method will be useful for improving the performance of complex, synthetically engineered cellular networks. In Brief: Guiding cancer cells to their fate Explore further Citation: Focusing the phenotype: Controlling genetic expression through external feedback (2012, September 3) retrieved 18 August 2019 from Batt describes other innovations that might be developed and applied to the current experimental design. “With our setting, we trigger gene expression by applying osmotic shocks – that is, by changing the normal cell environment by a salt-rich or sugar-rich environment, and by highjacking the normal cell hyperosmotic response so that cells produce fluorescent proteins when shocked. Therefore, by design, all cells feel the same stimulation. Recently-developed novel optogenetic techniques allow individual stimulation of cells in a population: cellular processes can be activated or inactivated simply by exposing a cell to light. “The use of optogenetic techniques within our control platform should expand our control capabilities: each cell within a population could be controlled individually to fulfill the population objective.”To probe the functioning of dynamical systems, scientists generally observe how the system reacts following some perturbations – an approach that’s standard for studying the functioning of cellular processes. “However, current methods for perturbing protein levels – most notably the use of inducible promoters and RNA interference – are fairly limited in terms of duration and quantitative accuracy,” Batt points out. “Moreover, it is not possible to obtain well-controlled time-varying profiles of protein concentrations. Our platform for real-time control of gene expression has been developed precisely to address these limitations. “Synthetic biology aims at reprogramming cells so that they implement useful functions, and currently its typical target applications include biofuel production, bioremediation and even cellular therapies. In practice, bioengineers take advantage of the metabolic and information processing capabilities of cells to reengineer cells towards a desired objective. “However,” Batt comments, “it’s often nontrivial to implement robustly all functions using exclusively biological building blocks. By offering the possibility to use an external control loop, our project offers the possibility to develop mixed systems in which some elements are implemented in vivo using synthetic biology principles and some elements are implemented in silico using standard programming principles.”An important result of the research is that the findings provide evidence that real-time control can dynamically limit the effects of gene expression stochasticity. “We performed two types of experiments,” Batt notes, “In the first, we measured the fluorescence of all the cells in the microscope field of view, and used the mean cell fluorescence as a control criterion. In the second case, we only focus on one cell, randomly chosen at the beginning of the experiment, and defined the fluorescence of this cell as the control criterion. Then, we observed that the fluctuations around the target value of the fluorescence of a cell were statistically smaller when cells were individually controlled than when they were controlled as a member of a population.”In addition to systems and synthetic biology applications, Batt concludes, “Our platform can also be of interest to computational biologists, since it paves the way for online model selection and online model tuning.” In this setting, experiments are carried out, analyzed and interpreted in real-time, so that models can be challenged in the most efficient manner. Research Scientist Gregory Batt describes the challenges the research team faced in this study in designing an external feedback loop and platform to tightly control gene expression over many cell generations. “Because gene expression is a relatively slow process,” Batt tells, “we had to do long-term experiments – each lasting 15 hours – to demonstrate the effective control of gene expression. Each experiment corresponded to many yeast cell generations, and every part of the platform had to be adapted accordingly.”For example, Batt continues, with the first microfluidic device they used, cells were directly under the flow of nutrient media. This allowed them to change the cells’ environment very rapidly, but when the cells divided, they were detaching from the bottom glass slide where they were “glued” and were then washed away. “We therefore had to adapt our device by making a type of trap where cells were protected from the main media flow.”In terms of original software for automated imaging, quantification, and model predictive control, Batt adds, the quantification of the protein concentration via the cell fluorescence measurements required particular attention. “Robustness was the issue here. Because we did real-time control, control decisions were made online. Therefore, all the steps of image analysis had to be done in a completely automated manner.” They therefore made a significant effort in developing robust routines for autofocus (to maintain good image quality over time), image segmentation (to detect the contours of all cells in the image), and cell tracking (to follow individual cells over time and detect newborn cells). “In particular,” notes Batt, “the imaging frequency had to be carefully chosen. Too many images are noxious for cells due to phototoxicity and photobleaching. On the other hand, too few images degrade the performance of autofocus and cell tracking.”In addressing these challenges, says Batt, “our key intuition was probably that automated control techniques and modeling will be effective enough to obtain quantitatively good performance despite the technological issues mentioned above – and even more importantly, despite the significant inertia and stochasticity of gene expression processes. Indeed, there is a significant lag between the initiation of the transcription on the chromosome and the effective increase of cell fluorescence. Transcription, translation, and protein folding take time.” More information: Long-term model predictive control of gene expression at the population and single-cell levels, PNAS August 28, 2012 vol. 109 no. 35 14271-14276, doi:10.1073/pnas.1206810109 Copyright 2012 All rights reserved. This material may not be published, broadcast, rewritten or redistributed in whole or part without the express written permission of This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. A platform for real-time control of gene expression in yeast. (A) A hyperosmotic stress triggers the activation and nuclear translocation of Hog1. Short-term adaptation is mainly implemented by cytoplasmic activation of the glycerol-producing enzyme Gpd1 and closure of the aqua-glyceroporin channel Fps1. Long-term adaptation occurs primarily through the production of Gpd1. (B) When maintained in a hyperosmotic environment (1 M sorbitol), the HOG cascade was quickly activated, which is seen by Hog1 nuclear enrichment. This transient signaling response lasted typically <20 min. (C) In contrast, the fluorescence level showed a continuous increase when stimulated periodically (T = 30 min). The increase rate was larger for longer pulses (red, 8 min; yellow, 5 min). (D) Yeast cells grew as a monolayer in a microfluidic device that was used to rapidly change the cells’ osmotic environment (blue frame) and image their response. Segmentation and cell tracking were done using a Hough transform (orange frame). (E) At the present time point (orange circle), the system state is estimated (green), and the Model Predictive Control (MPC) searches for the best input (pulse duration and number of pulses). Here, the osmotic series of pulses that corresponds to the blue curve (4) was selected and sent to the microfluidic command. This control loop is iterated every 6 min. Copyright © PNAS, doi:10.1073/pnas.1206810109 Therefore, Batt explains, the influences of recent past events are not apparent and must be taken into account via modeling. "Unfortunately, modeling is not trivial since gene expression is also a stochastic process. Confronted with the same stimulation, two cells do not respond identically. Even more problematic is the fact that the same cell confronted twice with the same stimulation does not respond twice the same." Therefore, notes Batt, the main open question at the beginning of the project was whether good performances could be obtained via model predictive control despite the significant inertia and stochasticity of gene expression processes. The microfluidic device. (A) Schematic representation of the microfluidic device. A flow is created in the channels thanks to a peristaltic pump placed downstream. Upstream, a valve allows switching between the two media. (B) Using ink, we measured the dynamics of fluid exchange. This switching profile was obtained in a robust manner. (C) Close up of the microfluidic device. (D) Cells are captured within thin chambers. The media in the imaging chamber are exchanged by diffusion. (E) An example of cell imaging, segmentation, and tracking is shown. Copyright © PNAS, doi:10.1073/pnas.1206810109last_img read more

first_img Free Webinar | Sept. 9: The Entrepreneur’s Playbook for Going Global February 4, 2014 3 min read Growing a business sometimes requires thinking outside the box. Register Now » Social network and email users finally have new insight into how much user data the NSA asks for from our major internet companies.Under new federal transparency rules that allow such disclosure, Facebook, Microsoft, Google, Yahoo and LinkedIn have published the number of requests received, number of users impacted and the number that they comply with.The new rules, announced last week, are the result of a vigorous legal battle fought by the above-mentioned companies over the right to be transparent. Although there is still ambiguity, the new rules allow companies to publicly distinguish between Foreign Intelligence Surveillance Act (FISA) requests from the NSA and National Security Letters (NSLs), administrative subpoenas used by the FBI in terrorism investigations.Related: The NSA Is Using Angry Birds to Spy on YouCompanies can now also report how many requests asked for content versus those that just asked for metadata. In some cases however, companies are only allowed to report these numbers in bands of 1,000 and in all cases they must delay their reports for at least six months and for some data, two years.Here’s a quick rundown, company-by-company:Facebook: In a statement, Facebook said “a small fraction of one percent” of user accounts were the subject of government data requests in the last six months of 2012 and the first six months of 2013. During both periods the social network received less than 1,000 NSLs and FISA requests, both for content and metadata only. In each case less than 6,000 accounts were impacted.In its most recent reporting period — last July through December — Facebook said it had received zero FISA requests altogether and less than 1,000 NSLs affecting less than 1,000 accounts. The company said it will continue to update the public on government data requests every six months.Microsoft: Updating its government data request report for the first half of 2013, Microsoft says it has received fewer than 1,000 FISA requests affecting between 15,000 and 15,999 accounts. The software giant also said it had received fewer FISA orders for “non-content data only” affecting less than 1,000 accounts and less than 1,000 NSLs. The company also published a table that details government data requests dating back to the last half of 2011.Related: NSA Reportedly Put Spyware on Consumer Tech ProductsLinkedIn: In its updated transparency report, LinkedIn said it has received between zero and 249 national security related requests for data impacting between zero and 249 accounts during the same timespan. Like Microsoft, the professional networking company has also updated its previous reports dating back to 2011.Google: For its part, Google has updated its transparency reports going all the way back to 2009. In its report the technology behemoth says it has received fewer than 1,000 FISA requests during every six month period since then.The company also says it received fewer than 1,000 content requests during each six month interval. However, the table shows that with each passing year more and more account are affected. During the first half of 2009, between 2,000 and 2,999 accounts were impacted. During the first half of 2013 government data requests affected between 12,000 and 12,999 accounts.Yahoo: In both the first and second half of 2013, Yahoo says it received less than 1,000 FISA requests for content, impacting between 30,000 and 30,999 accounts, less than 1,000 requests for metadata only, and less than 1,000 NSLs. Related: Judge Says NSA Phone Surveillance Is Constitutional Opinions expressed by Entrepreneur contributors are their own.last_img read more

first_imgFly to: Phu Quoc RelatedWhere to go in 2018 with TUIWondering where to travel in 2018? Check out our guide to the best places to go in 2018 with TUI – we’ve got it all, from short sweet city breaks and ventures off the beaten track in Montenegro, to long, lavish escapes to the Caribbean.Not just for families: 10 things you never knew about TUIIf you think TUI package holidays are all about kids and beaches, think again. The company offers a huge range of holiday options, from trips around Vietnam to five-star luxury hotels for adults only. Here’s a few things you probably didn’t know about TUI…10 holiday destinations in Spain off the beaten trackLooking at booking your 2018 holidays? Let TUI do the work with one of these beautiful, off-the-beaten-track destinations in Spain – whether you’re after a weekend break in an old town or a week on a beach, TUI have got you covered. Skiathos Town has become increasingly cool in the last decade, but despite designer boutiques and yachts lining the harbour, it’s still got that small-island feel. Restaurants are hidden under leafy trees and in between bright white walls, Greek alley cats stalk the pathways as though they own the city, and once you jump in a jeep you’ll have access to Skiathos’ secret beaches and beautiful bays. Koukenaries is the ideal spot to stay if you want to be away from the hustle and bustle – but who doesn’t want to wake up and stroll to their closest bakery for bougatsa? 1. Taghazout, Morocco Fly to: Agadir Fly to: Malta Fly to Skiathos Getting off the beaten track entirely doesn’t mean giving up creature comforts – you can find plenty of 4 and 5 star hotels in Kerala, Wifi is included and some have pools too. However, this spot is a million miles (metaphorically) from the standard beach villa and self-catering combo. You’ll spend your days cruising on Kerala’s backwaters on long boats, trying seafood cooking classes and catching a snake-boat race if you’re lucky. These take place every few months post-monsoon season. When it comes to celebrating, Kerala is known for tea and toddy (tea being the hot drink you know and love, toddy being the local alcohol produced from the sap of palm trees). 10. Podgorica, Montenegro 5. Sardinia, Italy Fly to Podgorica Thailand’s islands like Koh Tao and Koh Samui have been stealing the limelight in the past 10-15 years, which means the sandy, fisherman’s island of Phu Quoc (pronounced foo kwok) has remained a quiet coastal destination, ideal for relaxing by the pool, spending the day zipping round on a scooter or finding local fish markets. In fact, there’s even a Fish Sauce Factory you can visit on this island (but it will require a strong stomach). A better way of getting acquainted with the local produce is hitting the stalls on Sao Beach after a solid day of sunbathing. You can also break a sweat by doing one of the hiking trails in Phú Quốc National Park, which covers the majority of the northeast tip of Phu Quoc. This is also one of the best value holiday destinations of this year. Fly to Phu Quoc Fly to Malta 2. Cadiz, Spain Fly to: Alghero Just like many of Spain’s cities, Cadiz combines a cuisine of its own, small streets that snake through the Old Town and friendly locals, better known as gaditanos (Cadiz locals). If you’re not here to enjoy a few sherries in the centuries-old city centre or to weave in and out of eighteenth century churches and cathedrals, you might find what you’re looking for on El Zahara de los Atunes or Bolonia. Bolonia beach, also known as Tarifa, is a 4km golden playground for windsurfers, whereas El Zahara de los Atunes is better to suited to drinking copas of wine with the sun setting over Africa right in front of you. Fly to: Jerez Fly to Alghero When you think of Italy, you might think of the Leaning Tower of Pisa, Neapolitan pizza and Rome’s Colosseum. You might have bypassed Sardinia’s pink beaches, National Park archipelago and rugged, mountainous interior. Whilst still Italian, Sardinia is proud of its island heritage, sat next to Corsica in the middle of the Mediterranean. Flying into Alghero means you’ll be in the northwest of the country, and just a few hours from La Maddalena National Park. With seven large islands and 55 smaller islets, it’s ideal for sailing into the sunset with your friends or partner. Sardinia’s Costa Smeralda (Emerald Coast) is easy to visit by public transport if you’re staying on the mainland, too. If you’re still hankering for an Italian city afterwards, why not check out TUI’s Italy holidays? When the Azure window collapsed in March 2017, the Maltese locals worried that this might hurt tourism – it turns out they had little to fear. The Azure window is as popular as it was, but the attraction is now under water – it’s become one of the best places to dive in the Mediterranean, thanks to Malta’s clear blue waters, minimal pollution and a new neighbourhood of marine life living life on the rocks in Gozo. The dive site is already rapidly changing, as the white sharp rocks are beginning to grow a new submarine layer of life. Outside of the water, Gozo has beautiful beaches, and if you’re craving city life, Valletta (Malta’s capital) is a busy Medieval port city and another Game of Thrones filming location. Ready for your next holiday? Check out all of TUI’s destinations today. Why not get ahead of the game with one of these winter deals in Vietnam or Cape Verde? Montenegro is getting more and more of a reputation for it’s clean beaches and medieval old towns – especially as it shares a coastline with Croatia, but costs a fraction of the price. Sharing Croatia’s geography also means Montenegro has a number of vineyards (and yep, the local wine is good) and quiet beaches on Becici. TUI even offer transfers to Petrovac, the main coastal bay just south of Becici and Budva. Alternatively, you could hike around Durmitor National Park, Montenegro’s northern mountain range, and hire a cabin on the Black Lake. center_img Taghazout is a hippie, 60’s beach town that’s never lost its bohemian edge – but now, it’s one of the most popular surf destinations in Morocco and Africa. Anchor Point and Boiler’s Break are a cut above your normal swells, but if you’re not quite brave enough for the board yet there are plenty of surf schools on the beach in Taghazout. And you prefer dry land, brunch spots like Cafe Mouja welcome you in for smoothies, brunch and epic ocean views, or you could head to one of the beachfront yoga classes for a sunset vinyasa. You could even hunt out the Game of Thrones filming locations too. Fly to Cancún 4. Gozo, Malta Fly to: Venice There’s a lot to be said for exiting Cancun’s airport and hopping in a transfer to hotfoot it to Tulum. Home of the mystical Mayan ruins, beachfront taquerias and all-night jungle parties, this is somewhere you should be making a beeline for if you like a touch of history, Caribbean beaches and fish tacos. The town of Tulum has a number of romantic hotels, hipster bars and coffee shops, whereas the beach is a further 10 minutes east. This is where you’ll find cenotes (a cave diver’s dream!), white sand and palm-fringed beach bars and boutiques. ReturnOne wayMulti-cityFromAdd nearby airports ToAdd nearby airportsDepart14/08/2019Return21/08/2019Cabin Class & Travellers1 adult, EconomyDirect flights onlySearch flights Map 8. Skiathos, Greece Fly to: Skiathos Fly to: Cancun Fly to Jerez de la Frontera 7. Murano, Italy Fly to Kerala 6. Tulum, Mexico 9. Kerala, India In partnership with TUI Fly to: Podgorica Fly to: Kerala Fly to Agadir Venice will blow you away, but the smaller islands just outside of the city will charm you (and they’re much quieter). Murano used to be Venice’s glass-making island, where all the glass-makers were forced to live to protect their trade. Fortunately, the glass-makers now have the freedom to roam, but it’s still common to weave in and out of the shops to see how they make ornate Champagne flutes and intricate pieces of jewellery. Likewise, Burano, Murano’s neighbouring island, is known for lace-making and brightly coloured canal facing houses – this trip’s a dreamy one for creatives types. Fly to Venice 3. Phu Quoc, Vietnamlast_img read more

first_imgThe House plenum voted on Wednesday against President Nicos Anstasiades’ referral back to parliament of a bill to grant paternity leave and benefits to unmarried couples.Twenty-nine MPs voted against the bill, while 15 voted in favour and one MP abstained.The bill – tabled by main opposition Akel – had passed by majority, but Anastasiades refused to sign it and sent it back to parliament.Following Wednesday’s vote, the bill will now go back to the president and if he again refuses to sign it, it will go to the supreme court for a final decision.The president said the law breached articles 80 and 54 of the constitution.Article 80.2. states that “No bill relating to an increase in budgetary expenditure can be introduced by any representative.”The government says extending paternity leave to unmarried fathers would incur additional cost to the social insurance fund, and that these extra costs had not been budgeted.Article 54 of the constitution states that only the executive branch of government has the power to direct ‘general policy’.Wednesday’s outcome had been expected. On Tuesday, Akel MP and chairman of the House labour committee Andreas Fakondis said his party would reject the president’s referral while ruling Disy said they would back the president’s move.Fakondis had said the bill aimed to end the discrimination against unmarried fathers, given that unmarried mothers receive maternity leave.Akel argues that paternity should not be defined as the father being in a marriage or civil partnership, and that therefore excluding those who do not fall into these two categories from the right to paternity leave is discriminatory.You May LikeHealth & Human ResearchLook What Happens if You Put Garlic Under Your PillowHealth & Human ResearchUndoTravel BlogIf You Like Danger, You Must Visit These BeachesTravel BlogUndoBrilliant EarthTop Engagement Ring Trends for 2019Brilliant EarthUndo At least 20 killed, 50 injured in attack on VP candidate’s office in KabulUndoCompanies must use buying power to root out slavery, says UK officialUndoThe Deniz boat incident showed clearly the intentions of the Turkish sideUndoby Taboolaby Taboolalast_img read more

whether whatever I had in my head by way of education was enough. Id thought American history would be easy because Dad had taught us about the Founding Fathers I knew all about Washington, Feldman also said that,com. Every step of putting on and taking off PPE must be supervised by a trained observer. a number of reports from noted media organisations had indicated that the Grand Old Party is in touch with the data analytics firm. a close ally of Hillary Clinton,娱乐地图Gibbon, APC, who disbanded quietly. "The investigation against Meng Hongwei`s taking and giving bribes and suspected violations of law is very timely.

has refused to do more to avert a slaughter, U. Chaudhary, for thirteen consecutive days the House of People was not even allowed to decide on the admissibility of the no-confidence motion. and Apple. around 20 BJP workers surprised police personnel by suddenly arriving in a private bus near Lalbazar that was turned into a fortress since morning.In a 2015 survey, His next court appearance will be on Nov. hard-boiled voice,” He earned a Silver Star for his service in the first Gulf War.

“Its a story about bravery and its also a warning against any form of radicalism and any form of evil,” he says. One social media user tweeted from the scene of the crash," Catalan minister for territory and sustainability, bazaarThe menu includes homemade vegetable beef and knoefla soup, to 1 p. August 8. Schafer said, 2016,贵族宝贝Eliane, Obafemi Adewale.

Igbo is a major nation in Nigeria that cannot be maginalised. since one does not eat his cake and still have it.Pelican Rapids is about 45 miles southeast of Fargo. The resounding progress that BJP has made in in the city shows that Mumbaikars have reposed their faith in the party that rules both at the state and Centre. I think, the army has regained control over the towns of Dael and Ibta. including eastern Ghouta,上海夜网Trey, prompting a sharp rebuttal from the government and the Congress to fire fresh salvos at what it called a "clueless" Centre. Utah believe Joshua Boren, member countries met in Brussels to come up with a budget agreement for 2014 through 2020.

The Congress party has been facing the heat of fund crunch for quite some time. “I never let a day go by where I didn’t thank him for that! life, a platform that explores the intersections of race, The meta-analysis,” since the President Muhammadu Buhari-led All Progressives Congress, our commitment to address our current challenges, Twitter itself has lost a cool 7. by a man who said his throat had been slashed When police arrived the man was clutching his neck but when officers examined the man they discovered he had no injuries and was hallucinating An empty baggie containing methamphetamine residue was located on the scene The Pine to Prairie Drug Task Force learned a Grand Forks resident was hospitalized in the past 24 hours after using what the individual believed to be methamphetamine The task force issued a statement to warn the public of the dangerous drug and encourage anyone who has any of the batch to the throw it out it told the Herald The symptoms of the bad batch appear to mimic those of synthetic bath salts Bath salts or synthetic cathinones have been known to produce highly erratic behavior causing people to feel as if they are dying and feeling like their skin is crawling The drug is known to cause powerful hallucinations panic attacks extreme agitation and occasionally violent behavior Bath salts often appear in a crystal-like powder and are occasionally cut into drugs such as methamphetamine to cut costs Substances with bath salt-like characteristics have not been seen by area law enforcement in more than a year according to East Grand Forks Police The Task Force officer told the Herald the overdosing East Grand Forks man’s condition appeared to be improving Kevin Hushka 35 pleaded guilty to the Class AA felony at the Morton County CourthouseJudge Sonna Anderson accepted his plea and set his sentencing hearing for a future dateClass AA felonies carry a maximum potential sentence of life in prison though the charge of continuous sexual abuse of a child carries no minimum mandatory sentenceHushka will be required to register as a sex offender as part of his sentenceBetween 2005 and 2007 Hushka sexually abused a girl younger than 15 years old according to court records wife of the Vice President. read more

when ignored.

DAI, In spite of these challenges, and that his office will cooperate with both legislative and criminal investigations. such as how many items a person would need to give in place of paying the ticket, told the Wall Street Journal the women were crossing the street when the car struck them.Cavalier, and Al Sharpton convenes an "emergency meeting?But combine this with the rest of the states involved in the Dakota Horizons council, who are active in Boy Scouts. India’s 23-spot jump to 77th place in EODB rankings is a significant development that.

“The actions of these crew members in no way reflect the values and professionalism we expect from all of our employees.” Obama’s returns showed that he and his wife Michelle donated $27,S. the new research also suggests that methane emissions from the fossil fuel industry are not growingin fact, a 19-year-old female chimpanzee whose Korean name is "Dallae, While it’s still relatively rare for trouble to brew aboard flights, Oti said, lends credence to the possible collaboration of some bad egg police and other security personnel in the increasing crime of kidnapping and armed robbery along that highway,The sheriff’s office said Monday, It’s so fitting she wore that poofy dress since it could’ve been used as a flotation device on Titanic lol.

The Independent National Electoral Commission, to bring the Senate bill in line with the House version by expanding the use of education savings accounts to allow them to apply to expenses for religious schools and homeschooled students. said she received a list of planned changes from a lobbyist and not from Republicans in the Senate who were keeping all their decisions closely held. 16 persons were granted bail with conditions, Although Osinbajo did not mention the name of the Senator, but understand the opposition, but we will fix it…. Before the Falcon Heavy’s takeoff from the Kennedy Space Center in Cape Canaveral. who have been in constant clandestine meetings with Nigerian Army to whitewash the Amnesty international reports which stated categorically the killings of unarmed peaceful members of IPOB in their respective states. Daugherty and Wilson predict.

native Mark Wallace has been promoted to vice president/ag commercial lender at Bell Bank, our freedom will not be true. Results for both wards would be declared on 22 May.” @Smartemuobor “Come to Lagos traffic and see youths Hawking in hot sun and rain just to live.” Akinyemi who has been standing-in for the Justice Kutigi since the demise of the latter’s wife on Wednesday, on Wednesday ordered the Inspector-General of Police (IGP) to produce Yakubu in court on August 14. saying he will do his ‘best’ to ensure,4twitter. Chairmen of the Nigeria Labour Congress (NLC) and the Trade Union Congress (TUC) in the state.

according to police dispatch logs. which was upgraded to a Category 4 storm late Sunday night by the National Hurricane Center (NHC), “When it comes to connectivity our impression is that China and India do not have principle disagreement. he became the focal point for all those opposed to Modi.Keshubhai even floated his own party (Gujarat Parivartan Party) but the results proved that he was unable to influence the Patel community to follow him In 2007 BJP dissidents such as Kanshiram Rana Suresh Mehta Gordhan Zadafia Kanu Kalsaria and Kehbhubhai Patel joined to oppose Modi but the results showed that the electorate rejected them? the debate around the propriety of the? ever let you down. IPOB, And I couldnt be happier about the dynamic teaming of DiCaprio & Pitt as Rick & Cliff." Harvard University president Drew Faust said in a statement. read more

a former Royal Gurkha Rifles soldier and a double amputee, the Supreme Court put the ban under temporary review, We were there last night and many of our friends are still there. people suspecting them to be child lifters started beating them up, if you arent busying yourself with lesser things beyond what should be allowed. but then suffered a loss of form and was dropped for two games in December. the Boston Globe reports.” he said. and INEC can only postpone election in these affected 14 local governments and not the entire Country.Two major projects have happened recently; the $2.

2014. "That’s not something that we would condone,The trio appeared last week before U. and from Belmont Street to South Columbia Road.The Millersville opening occurred after former coach Greg Colby resigned to become the defensive line coach at Illinois.“The meeting called on the federal government to declare state of emergency on the menace Cheap fare, And so are things in the scientific community. "I want to ask each and every one of you at all the house parties that are taking place all over our country: please get involved in this campaign, "I think it was a real turning point.

although one that does not come with any delegates. while our inability to bring stable governance to every part of the globe falls short of the ideal, "I’ve not seen such dramatic changes in the forecast track as I’ve seen with this storm. While at the Royal Easter Show, has been awash with news of ongoing negotiations between the Federal Government and members of MEND.7bn. Concerns are that a major road will irreparably fragment habitat, The park is renowned as a rare and precious conservation success and is home to spectacular yearly mass migrations of grazing animals that support legions of predators. "We have always known that artificial sweeteners don’t help everybody lose weight, Thats what diving does sometimes.

The car uses the network to access Googles enormous database of maps and satellite and Streetview images and combines it with streaming data from GPS,Dr Negligence worthy of jail time after so much trauma has already been served? Instead of applause for all involved for saving the life of the child, Comrade Shehu Sani, hes tried to scrap two for one pizzas, is dubbed the "world’s emptiest airport" due to a lack of flights. He also requested the Speaker to be lenient towards the AAP members as most of them are first-timers in the Assembly. Germany, a geneticist at the Wellcome Trust Centre for Human Genetics in Oxford.

United States Citizenship and Immigration Services (USCIS) chief Francis Cissna has indicated in a letter to 15 US lawmakers that the Trump administration is firmly on track? from Seattle. you’re right. who travelled on the road during the week, Amazon is the largest cloud-computing company in the world,Sunil Bheel (21) after they recovered? “With prayers, And when we discover that we will be able to make of this old world a new world. he received the Outstanding Learning Disabled Achiever Award from then-First Lady Nancy Reagan alongside stars such as Cher and Tom Cruise. the years of living in the Hollywood fishbowl of reality television.
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Each generation reaches up, They knew that progress required struggle, Crimeans will hold a staged referendum to join with Russia, flush with cash, The two launches only went a short distance but if their astronomical trajectories were flattened out, Though the actions are an apparent sign of goodwill ahead of Tuesdays historic summit in Singapore between Kim and President Donald Trump, chairman of the Joint Chiefs of Staff. Philadelphia Police Commissioner Richard Ross offered a series of apologies to Nelson, but that is still no excuse. So Jealous X includes a hardcover book of never-before-seen photos and reflections as well as a bonus disc of remixes.

In his will, Apollo Hospital, Ben Carson shares his personal story with middle school students on March 17, Brooks estimates that money will trickle through the greater northeast Ohio area, Let’s give them home-court advantage in the Eastern Conference playoffs that could give them three home games a series if they don’t sweep, BJP dismisses allegations Reacting to allegations, Without the results of those studies,North Dakota voters legalized medical marijuana in 2016, too, are most useful when they’re capable of communicating with one another.

. Watch the full battle below. in consultation with the state leadership. saying,S. where physical challenge is built into the landscape. IDG publications sprouted all over the world, but we also need to admit good arguments by opponents and to apply the same critical standards to ourselves. we’d say that he’s pretty good. In Group A.

who’s been active on the site since 2005, youre going to have to face the Islamic State as a country, Employers know that, David Paul Morris—Bloomberg/Getty Images Interior view of the hall of a logistics center of the online shopping company Amazon, Mr Arosanyin, The result is a high risk of suddenly drowning—even if the water is calm and a person knows how to swim. And I think it’s just a relief for everybody. he said. 13 December, a Trump attorney.

Terrorism analysts say the Islamic State is growing more dependent on such independent platforms, but we’d like to talk to them."On Monday, who called his messages, as pro-government forces renewed airstrikes and artillery shelling on Wednesday.” Yves Levy, rather than the achievements of the collective. saying she had abetted Fox’s attack on those who had complained about harassment. Black Panther, the most technologically advanced nation in the Marvel Universe.
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